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GrainGenes Marker Report: Xwmc104

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Locus
Xwmc104
Type
Microsatellite
Chromosome
1A
Map
Wheat-Composite2004-1A29
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Probe
WMC104
Possible Orthologs
Xwmc104-1A
Xwmc104-6B
wmc104
wmc104-6B



Probe for Locus
WMC104
Locus
Xwmc104-1A
Xwmc104-6B
Xwmc104
wmc104
wmc104-6B
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 7d5
Donor: Somers, Agriculture Canada
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 132 bases located on 6B, 143 bases located on 6B, 95 bases located on 6B
Trimmed to insert (Rsa1 at 3'). (GT)31 44 to 105. (2 loci both polymorphic?). Lower locus is 7D - linked to preharvest sprouting in SPR8198 x HD2329 ( Roy et al Theor Appl Gene 99 336-340 (1999))
Type
PCR
SSR
PCR primers
Forward TcTcccTcATTAgAgTTgTccA at 21 length 22 bases, Tm 61
Reverse ATgcAAgTTTAgAgcAAcAccA at 139 length 22 bases, Tm 61
SSR size
140 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC104
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06

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