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GrainGenes Marker Report: Xwmc120

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Locus
Xwmc120
Type
Microsatellite
Chromosome
1A
Map
Ta-NxW-1A67.2
Wheat-Composite2004-1A52
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Wheat, Nanda2419 x Wangshuibai
Probe
WMC120
Possible Orthologs
Xwmc120-1A
wmc120
wmc120-1A



Probe for Locus
WMC120
Locus
Xwmc120-1A
Xwmc120
wmc120
wmc120-1A
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 8d12
Donor: Flore, Verneuil
AGR199. Matches 8c12. Multiple inserts (2) joined at 314 to 331 by linkers. Trimmed to insert at 5' end and Rsa1 at 3' end. (CA)8 379 to 394, (GA)14 228 to 255, (GT)8 130 to 145. Primers designed to second insert. (2 loci - both polymorphic?)
Type
PCR
SSR
PCR primers
Forward ggAgATgAgAAgggggTcAggA at 344 length 22 bases, Tm 68
Reverse ccAggAgAccAggTTgcAgAAg at 483 length 22 bases, Tm 67
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC120
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06