Query (optional)   in Class  

GrainGenes Marker Report: Xwmc173e

[Submit comment/correction]

Locus
Xwmc173e
Type
Microsatellite
Chromosome
5A
Map
Wheat-Composite2004-5A0
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Probe
WMC173
Possible Orthologs
Xwmc173-1D
[ Show all 11 ]



Probe for Locus
WMC173
Locus
Xwmc173-4A.1
[ Show all 12 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 9f3.2
Donor: Bernard, Clermont-Ferrand
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 263-279 bases located on 4A
Trimmed to Rsa1 at both ends. Multiple inserts (2) joined at 382-399 by linkers. (CA)13 705 to 730, (GA)8 121 to 136. Primers shown are for second insert.
Type
PCR
SSR
PCR primers
Forward TgcAgTTgcggATccTTgA at 532 length 19 bases, Tm 66
Reverse TAAccAAgcAgcAcgTATT at 786 length 19 bases, Tm 56
SSR size
273 bases expected, Chinese Spring
Amplification Conditions
51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence
WMC173
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.