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GrainGenes Marker Report: Xwmc179c

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Locus
Xwmc179c
Type
Microsatellite
Chromosome
2B
Map
Wheat-Composite2004-2B71
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Probe
WMC179
Possible Orthologs
Xwmc179-2B
[ Show all 17 ]



Probe for Locus
WMC179
Locus
Xwmc179.1-1D
[ Show all 18 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 9h5
Donor: Bernard, Clermont-Ferrand
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 132 bases located on 2A, 136 bases located on 1D, 142 bases located on 7A, 198 bases located on 6A, 237 bases located on 2B, 261 bases located on 3D, 365 bases located on 6A
Trimmed to insert at both ends. (GA)17 415 to 448. Bases 1 to 507 show homology to 15h1 bases 1 to 507 (507 in longest run, maxScore = 414). 9h5 is a duplicate of 15h1. 9h5 has priority.
Type
PCR
SSR
PCR primers
Forward cATggTggccATgAgTggAggT at 301 length 22 bases, Tm 70
Reverse cATgATcTTgcgTgTgcgTAgg at 480 length 22 bases, Tm 68
SSR size
201 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence
WMC179
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06