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GrainGenes Marker Report: Xwmc188

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Locus
Xwmc188
Type
Microsatellite
Chromosome
5B
Map
Wheat-Composite2004-5B32
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Map Data
Wheat, Composite, 2004
Probe
WMC188



Probe for Locus
WMC188
Locus
Xwmc188
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 4b8
Donor: Morell, Canberra
Trimmed to insert at both ends (removing Ns). Multiple inserts (2) in this clone, joined at 277-294 by linkers. (CA)9 137 to 154. Second insert is a member of 1g1 family.
Type
PCR
SSR
PCR primers
Forward cgcgTggAATTAgATgAcccTT at 3 length 22 bases, Tm 66
Reverse ccTTTgcTTccgATcAAATgTg at 237 length 22 bases, Tm 65
SSR size
256 bases expected, Chinese Spring
Amplification Conditions
tested 61C anneal, no product; 51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence
WMC188
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06

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