Query (optional)   in Class  

GrainGenes Marker Report: Xwmc238

[Submit comment/correction]

Locus
Xwmc238
Type
Microsatellite
Map
Ta-NxW-4B23.9
[ Show Nearby Loci ]
Map Data
Wheat, Nanda2419 x Wangshuibai
Probe
WMC238
Possible Orthologs
Xwmc238-4B
wmc238



Probe for Locus
WMC238
Locus
Xwmc238
Xwmc238-4B
wmc238
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 5f2
Donor: Sharp, Camden
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 181 bases located on 4B, 227 bases located on 4B
Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 87-270 match part of 5B12, and part of 5g10. (CA)22 96 to 139. Reverse primer is outside of homology with 5b12.
Type
PCR
SSR
PCR primers
Forward TcTTccTgcTTAcccAAAcAcA at 70 length 22 bases, Tm 64
Reverse TAcTgggggATcgTggATgAcA at 272 length 22 bases, Tm 69
SSR size
224 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC238
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.