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GrainGenes Marker Report: Xwmc254-4B

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Locus
Xwmc254-4B
Type
Microsatellite
Chromosome
4B
Map
Ta-SSR-2004-4B23.3
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC254
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc254
[ Show all 7 ]



Probe for Locus
WMC254
Locus
Xwmc254-1A
[ Show all 8 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 4b2
Donor: Gupta, Meerut
No trimming done to sequence. (CA)28 31 to 86. Forward primer is not ideal.
Type
PCR
SSR
PCR primers
Forward AgTAATcTggTccTcTcTTcTTcT at 2 length 24 bases, Tm 58
Reverse AggTAATcTccgAgTgcAcTTcAT at 171 length 24 bases, Tm 64
SSR size
193 bases expected, Chinese Spring
Amplification Conditions
51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence
WMC254
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06

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