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GrainGenes Marker Report: Xwmc28

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Locus
Xwmc28
Type
Microsatellite
Chromosome
5B
Map
Ta-NxW-5B217.1
Wheat-Composite2004-5B125
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Wheat, Nanda2419 x Wangshuibai
Probe
WMC28
Possible Orthologs
Xwmc28-5B
wmc28



Probe for Locus
WMC28
Locus
Xwmc28-5B
Xwmc28
wmc28
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 2g9
Donor: Wang, Utah
One small (CA) and one large (T)(CA) compound.(GT)29 132 to 189
Type
PCR
SSR
PCR primers
Forward ATcAcgcATgTcTgcTATgTAT at 42 length 22 bases, Tm 59.0
Reverse ATTAgAccATgAAgAcgTgTAT at 208 length 22 bases, Tm 55.0
SSR size
188 bases expected, Chinese Spring
Amplification Conditions
51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence
WMC28
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06