GrainGenes Marker Report: Xwmc396-7B

Locus

Type

Microsatellite

Chromosome

Map

Kofa/UC1113-7B	85.3
Louise/Penawawa-7B	27.7
Ta-SSR-2004-7B	67.3

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Map Data

Durum wheat, Kofa x UC1113

Wheat, Consensus SSR, 2004

Wheat, Louise x Penawawa

Probe

WMC396

Reference

Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.

Possible Orthologs

Probe for Locus

Locus

Reference

Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.

General Remarks

Wheat Microsatellite Consortium Project 1, Plate reference 10g12

Donor: Jacquemin, Gembloux

Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 149 bases located on 7B

Trimmed to Rsa1 at 5' end, 3' end not located. (GT)14 122 to 149. Bases 73 to 250 show homology to 14h11 bases 1 to 178 (complement) (178 in longest run, maxScore = 119). Duplicate of 14h11, 10g12 has priority.

Type

PCR

SSR

PCR primers

Forward TgcAcTgTTTTAccTTcAcggA at 27 length 22 bases, Tm 65
Reverse cAAAgcAAgAAccAgAgccAcT at 151 length 22 bases, Tm 65

SSR size

146 bases expected, Chinese Spring

Amplification Conditions

61C anneal, polymorphic on acrylamide.

Sequence

WMC396

Source Species

Triticum aestivum

Data Source

Somers, Daryl J.	2004.06
Isaac, Peter G.	2004.06


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