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GrainGenes Marker Report: Xwmc398b-6A

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Locus
Xwmc398b-6A
Type
Microsatellite
Map
CS/SQ1-6A30.8
[ Show Nearby Loci ]
Map Data
Wheat, Chinese Spring x SQ1
Probe
WMC398
Possible Orthologs
Xwmc398
[ Show all 7 ]



Probe for Locus
WMC398
Locus
Xwmc398b-6A
[ Show all 8 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 22c7
Donor: Romestant, RAGT
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 156 bases located on 6B
Trimmed to insert at 5' end, corrupted 3' end removed as cleanly as possible. Bases 149 to 198 show homology to 11a8 bases 348 to 397 (50 in longest run, maxScore = 42). (GT)14 32 to 59. Note that the 3' primer is located close to corrupted sequence of vector.
Type
PCR
SSR
PCR primers
Forward ggAgATTgAccgAgTggAT at 1 length 19 bases, Tm 60
Reverse cgTgAgAgcggTTcTTTg at 136 length 18 bases, Tm 60
SSR size
152 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic on acrylamide.
Sequence
WMC398
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06

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