GrainGenes Marker Report: Xwmc435-7B

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Locus

Xwmc435-7B

Type

Microsatellite

Chromosome

Map

Ta-SSR-2004-7B

64.8

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Map Data

Wheat, Consensus SSR, 2004

Probe

WMC435

Reference

Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.

Possible Orthologs

Xwmc435

[ Show all 10 ]

Probe for Locus

WMC435

Locus

Xwmc435

[ Show all 11 ]

Reference

Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.

General Remarks

Wheat Microsatellite Consortium Project 1, Plate reference 24f4

Donor: ITCF

Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 147 bases located on 5A

Trimmed to insert at both ends. Bases 66 to 135 (complement) show homology to 5a4 bases 30 to 99 (70 in longest run, maxScore = 91) Homology on one side of microsatellite, forward primer has no homology to 5a4, reverse primer has a single mismatch. (GT)10 35 to 54,

Type

PCR

SSR

PCR primers

Forward gcAcTATAcTTATTggATTgTcA at 4 length 23 bases, Tm 55
Reverse cATggTATcccTAgTAAgTTTTT at 127 length 23 bases, Tm 55

SSR size

146 bases expected, Chinese Spring

Amplification Conditions

51C anneal, smear.

Sequence

WMC435

Source Species

Triticum aestivum

Data Source

Somers, Daryl J.	2004.06
Isaac, Peter G.	2004.06


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