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GrainGenes Marker Report: Xwmc463-7D

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Locus
Xwmc463-7D
Type
Microsatellite
Chromosome
7D
Map
CS/SQ1-7D41.1
Ta-SSR-2004-7D71.7
[ Show Nearby Loci ]
Map Data
Wheat, Chinese Spring x SQ1
Wheat, Consensus SSR, 2004
Probe
WMC463
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
wmc463
Xwmc463



Probe for Locus
WMC463
Locus
Xwmc463-7D
SrTA10171
wmc463
Xwmc463
Reference
ReferenceOlson EL et al. (2013) Introgression of stem rust resistance gene SrTA10187 and SrTA10171 from Aegilops tauschii to wheat Theoretical and Applied Genetics 126:2477-2484.
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
ReferenceMAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Stem Rust Resistance. SrTA10171 MAS Wheat. Marker Assisted Selection in Wheat.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 21a8
Donor: Dupont
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 153 bases located on 7D
Trimmed to insert at 5' end and to Ssp1 at 3' end. (GT)26 184 to 235.
Type
PCR
SSR
PCR primers
Forward gATTgTATAgTcggTTAccccT at 121 length 22 bases, Tm 58
Reverse ATTAgTgcccTccATAATTgTg at 251 length 22 bases, Tm 60
SSR size
152 bases expected, Chinese Spring
Amplification Conditions
51C anneal, polymorphic on acrylamide (115, 152-156 bases).
Sequence
WMC463
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06

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