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GrainGenes Marker Report: Xwmc491b

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Locus
Xwmc491b
Type
Microsatellite
Chromosome
4A
Map
Wheat-Composite2004-4A54
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Probe
WMC491
Possible Orthologs
Xwmc491-4A
[ Show all 7 ]



Probe for Locus
WMC491
Locus
Xwmc491-4A
[ Show all 8 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 25a9
Donor: Serasem
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 194 bases located on 4A
Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 257 to 269 (complement) show homology to 12d2 bases 27 to 39 (13 in longest run, maxScore = 33). Bases 112 to 131 show homology to 25c11 bases 73 to 92 (20 in longest run, maxScore = 46). (GT)28 151 to 206, (GT)5 251 to 260.
Type
PCR
SSR
PCR primers
Forward ggTAAAAcTTcgTgTcccTTgc at 56 length 22 bases, Tm 64
Reverse TAgTTgcgAgTcggTAgTcTgc at 226 length 22 bases, Tm 64
SSR size
192 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic on acrylamide (116-126 bases, 198-202 bases).
Sequence
WMC491
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06