GrainGenes Marker Report: Xwmc546-7B.2

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Locus

Xwmc546-7B.2

Type

Microsatellite

Chromosome

Map

Ta-SSR-2004-7B

56.4

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Map Data

Wheat, Consensus SSR, 2004

Probe

WMC546

Reference

Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.

Possible Orthologs

Probe for Locus

Locus

[ Show all 6 ]

Reference

Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.

General Remarks

Wheat Microsatellite Consortium Project 1, Plate reference 26g7

Donor: Momont

Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 172 to 202 (complement) show homology to 22d9 bases 22 to 52 (31 in longest run, maxScore = 58). Reverse has 4 mismatches to 22d9, forward primer no obvious match. (CA)9 95 to 112, (CA)8 115 to 130.

Type

PCR

SSR

PCR primers

Forward cggcTAAAATcgTAcAcTAcAcA at 12 length 23 bases, Tm 61
Reverse cTcAcTTgcAcgATTTcccTAT at 141 length 22 bases, Tm 62

SSR size

151 bases expected, Chinese Spring

Amplification Conditions

61C anneal, polymorphic on acrylamide (110-170 bases, complex).

Sequence

WMC546

Source Species

Triticum aestivum

Data Source

Somers, Daryl J.	2004.06
Isaac, Peter G.	2004.06


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