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GrainGenes Marker Report: Xwmc70

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Locus
Xwmc70
Type
Microsatellite
Chromosome
7B
Map
Wheat-Composite2004-7B91
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Probe
WMC70
Possible Orthologs
Xwmc70-7B
wmc70



Probe for Locus
WMC70
Locus
Xwmc70
Xwmc70-7B
wmc70
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 1d12
Donor: Waugh, SCRI
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 220 bases located on 7B
(CA)22 96 to 139, (CA)24 141 to 188. (1 polymorphic locus). Close relative (duplicate?) of 1d2, 1G4 - do they detect at least 1 common locus?
Type
PCR
SSR
PCR primers
Forward ggggAgcAcccTcTATTgTcTA at 22 length 22 bases, Tm 63.0
Reverse TAATgcTcccAggAgAgAgTcg at 213 length 22 bases, Tm 65.0
SSR size
213 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence
WMC70
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06