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GrainGenes Marker Report: bcd1261a

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Locus
bcd1261a
Type
RFLP
Map
Oat-KxO/2003-110.7
[ Show Nearby Loci ]
Map Data
Oat, KxO, 2003
Probe
BCD1261
Homology
AF236863_1e-value: 9.3(AF236863) dihydrofolate reductase [Lactococcus lactis]
T32976e-value: 1.2hypothetical protein F57H12.3 - Caenorhabditis elegans
T32976e-value: 0.76hypothetical protein F57H12.3 - Caenorhabditis elegans
Remark
Marker placement value = 16.40 cM on map Oat-KxO/2003-1
Marker is not a framework marker on map Oat-KxO/2003-1
Possible Orthologs
bcd1261
[ Show all 15 ]



Probe for Locus
BCD1261
Locus
Xbcd1261-1
[ Show all 16 ]
Synonym
SynonymHAR35_32749
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Type
cDNA
Sequence
BE438635
[ Show all 6 ]
Polymorphism
BCD1261 DraI
BCD1261 XbaI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
PCR size
0.8
Clone Vector
pBluSK
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD1261 KO autoradiogram
BCD1261 SO autoradiogram
Data Source
Sorrells, Mark E.96.07