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GrainGenes Marker Report: bcd1414a

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Locus
bcd1414a
Type
RFLP
Map
Oat-2004-TxM_10
Oat-KxO/2003-332.84
[ Show Nearby Loci ]
Map Data
Oat, KxO, 2003
Oat-2004-TxM
Probe
BCD1414
Homology
THN5_HORVUe-value: 5e-10LEAF-SPECIFIC THIONIN PRECURSOR (CLONES PKG1348, PKG1940, PKG2872 AND DG3)
Remark
Marker placement value = 4.82 cM on map Oat-KxO/2003-33
Marker is not a framework marker on map Oat-KxO/2003-33
Possible Orthologs
bcd1414
[ Show all 7 ]



Probe for Locus
BCD1414
Locus
bcd1414
[ Show all 8 ]
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Type
cDNA
Sequence
BE438926
BCD1414_WHE04B07.s1
Polymorphism
BCD1414 DraI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
PCR size
0.9
Clone Vector
pBluSK
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD1414 KO autoradiogram
Data Source
Sorrells, Mark E.96.07

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