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GrainGenes Marker Report: cdo1414a

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Locus
cdo1414a
Type
RFLP
Map
Oat-KxO/2003-11_41+207.1
[ Show Nearby Loci ]
Map Data
Oat, KxO, 2003
Probe
CDO1414
Remark
Marker placement value = 2.76 cM on map Oat-KxO/2003-11_41+20
Marker is not a framework marker on map Oat-KxO/2003-11_41+20
Possible Orthologs
cdo1414b
[ Show all 10 ]



Probe for Locus
CDO1414
Locus
Xcdo1414B
[ Show all 11 ]
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
Type
cDNA
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
PCR size
1.5
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
Clone Authority
Sorrells, Mark E.
Image
CDO1414 KO autoradiogram
Data Source
Sorrells, Mark E.96.07