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GrainGenes Marker Report: cdo1461b

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Locus
cdo1461b
Type
RFLP
Map Data
Oat, KxO, 2003
Probe
CDO1461
Homology
S19224e-value: 2e-10ribosomal protein L10.e, cytosolic - rice (fragment)
Remark
Marker placement value = 0.00 cM on the Oat-KxO/2003 maps
Marker is unlinked on the Oat-KxO/2003 maps
Possible Orthologs
cdo1461
Xcdo1461
CDO1461
CDO1461B



Probe for Locus
CDO1461
Locus
Xcdo1461
cdo1461
cdo1461b
CDO1461
CDO1461B
Synonym
SynonymHAR35_264
HAR35_49601
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
Type
cDNA
Sequence
BE439309
CDO1461_WHE05H04.s1
Polymorphism
CDO1461 EcoRI
Source Species
Avena sativa
Source Germplasm
Brooks (oat)
Source Tissue
etiolated leaf
PCR size
0.9
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO1461 KO autoradiogram
Data Source
Sorrells, Mark E.96.07

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