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GrainGenes Marker Report: cdo358edi

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Locus
cdo358edi
Type
RFLP
Map
Oat-KxO/2003-1715.6
[ Show Nearby Loci ]
Map Data
Oat, KxO, 2003
Probe
CDO358
Homology
BAA77727.1e-value: 6.5(AB008676) flippase [Escherichia coli]
RLA0_MAIZEe-value: 8e-1160S ACIDIC RIBOSOMAL PROTEIN P0
Remark
Marker placement value = 1.91 cM on map Oat-KxO/2003-17
Marker is not a framework marker on map Oat-KxO/2003-17
The 'di' in this locus name indicates that Dra1 was used to generate the polymorphism in this population
Possible Orthologs
CDO358
[ Show all 15 ]



Probe for Locus
CDO358
Locus
CDO358
[ Show all 16 ]
Reference
ReferenceWight CP et al. (2004) Discovery, localization, and sequence characterization of molecular markers for the crown rust resistance genes Pc38, Pc39, and Pc48 in cultivated oat (Avena sativa L.) Molecular Breeding 14:349-361.
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
Type
cDNA
Sequence
BE439059
BE439110
CDO358_WHE2F0051
CDO358_WHE2F0052
Linkage Group
[b 1S]
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
1.4
PCR size
1.4
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Data Source
Sorrells, Mark E.96.07
ATMI92.09