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GrainGenes Marker Report: cdo419d

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Locus
cdo419d
Type
RFLP
Map
Oat-KxO/2003-21+46_31+4021.5
[ Show Nearby Loci ]
Map Data
Oat, KxO, 2003
Probe
CDO419
Remark
Marker placement value = 6.38 cM on map Oat-KxO/2003-21+46_31+40
Marker is not a framework marker on map Oat-KxO/2003-21+46_31+40
Possible Orthologs
CDO0419
[ Show all 24 ]



Probe for Locus
CDO419
Locus
CDO419c
[ Show all 24 ]
Synonym
SynonymHAR35_34450
HAR35_36597
HAR35_432
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
Type
cDNA
Sequence
BE439269
CDO419_WHE04F06.s1
Polymorphism
CDO419 EcoRI
Linkage Group
[b 2LH, 6H]
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
1.8
PCR size
1.8
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO419 KO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09