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GrainGenes Marker Report: cdo506a

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Locus
cdo506a
Type
RFLP
Chromosome
2H
Map
Barley-consensus-2003-2H49.7
Oat-KxO/2003-7_10_28173
[ Show Nearby Loci ]
Map Data
Barley consensus 2003
Oat, KxO, 2003
Probe
CDO506
Homology
A83106e-value: 8.4hypothetical protein PA4320 [imported] - Pseudomonas aeruginosa (strain PAO1)
AAH04729e-value: 8e-08(BC004729) RIKEN cDNA 1810063P04 gene [Mus musculus]
Data Source
Langridge, Peter J.
Remark
Marker placement value = 1.97 cM on map Oat-KxO/2003-7_10_28
Marker is not a framework marker on map Oat-KxO/2003-7_10_28
Possible Orthologs
CDO506
[ Show all 12 ]



Probe for Locus
CDO506
Locus
CDO506
[ Show all 16 ]
Synonym
SynonymHAR35_17150
HAR35_41262
External Databases
DDBJ
EMBL
GenBank
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
OatUni-ZAPXR
Type
cDNA
STS
PCR primers
TCA TTC AGA GGA AGA ACT GC
ATA GTG GAG CAA AGA CTG TC
Sequence
BE439286
BE439294
CDO506_WHE04G12.s1
CDO506
Polymorphism
CDO506 EcoRI
CDO506 EcoRV
Linkage Group
[b 7L]
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
1.2
1.4
0.6
Clone Vector
pBluSK
BS-SK-
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Blake, Tom
Image
CDO506 BROWS autoradiogram
CDO506 KO autoradiogram
CDO506 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
Blake, Tom95.08.24
ATMI92.09