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GrainGenes Marker Report: cdo708b

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Locus
cdo708b
Type
RFLP
Map
Oat-KxO/2003-22_44+18112
Oat-OxT-462.1
[ Show Nearby Loci ]
Map Data
Oat, KxO, 2003
Oat, OxT
Probe
CDO708
Remark
Marker placement value = 0.00 cM on map Oat-KxO/2003-22_44+18
Marker is a framework marker on map Oat-KxO/2003-22_44+18
Possible Orthologs
CDO0708
[ Show all 7 ]



Probe for Locus
CDO708
Locus
Xcdo708
[ Show all 8 ]
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
Type
cDNA
Sequence
AY245605
CDO708
Polymorphism
CDO708 EcoRV
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
PCR size
1.2
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
Clone Authority
Sorrells, Mark E.
Image
CDO708 KO autoradiogram
Data Source
Sorrells, Mark E.96.07