GrainGenes Marker Report: gwm389

Locus

Type

Microsatellite

Map

Durum Wheat, NSGC Lr 3B	0
[ Show all 6 ]

Map Data

3B Physical

Probe

WMS389

Possible Orthologs

Xgwm389

Probe for Locus

WMS389

Locus

Xgwm389-3B

[ Show all 6 ]

Reference

Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.

General Remarks

Insert contains repeat (CT)14(GT)16

PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.

Type

PCR

SSR

PCR primers

5' ATCATGTCGATCTCCTTGACG 3'
5' TGCCATGCACATTAGCAGAT 3'

Amplification Conditions

3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C

Polymorphism

Source Species

Source Germplasm

Data Source

98.09.15


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