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GrainGenes Marker Report: pbcd175

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Locus
pbcd175
Type
STS
Map
Oat-KxO/2003-11_41+203.38
[ Show Nearby Loci ]
Map Data
Oat, KxO, 2003
Probe
BCD175
Homology
JW0059e-value: 9.4mtprd protein - mouse
AC007190_12e-value: 1.4(AC007190) F23N19.15 [Arabidopsis thaliana]
Remark
Marker placement value = 7.96 cM on map Oat-KxO/2003-11_41+20
Marker is not a framework marker on map Oat-KxO/2003-11_41+20
Possible Orthologs
BCD175
[ Show all 9 ]



Probe for Locus
BCD175
Locus
BCD175
[ Show all 9 ]
Synonym
SynonymHAR35_16859
HAR35_16860
HAR35_36251
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Bar.LambZAPII
Type
cDNA
Sequence
BE438667
[ Show all 6 ]
Polymorphism
BCD175 EcoRI
Linkage Group
[b 2S]
[b 2S]
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
0.7
PCR size
0.7
Clone Vector
pBluSK
pBluSK-
BS-SK-
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD175 BROWSSM autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09

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