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GrainGenes Marker Report: Ebmac0788

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Locus
Ebmac0788
Type
Microsatellite
Synonym
SynonymEBmac788
Chromosome
4H
Map
[ Hide all but 1 of 10 ]
Barley, B73xCPI-4H101
Hordeum-Consensus2005-SNP-4H99.515
Hordeum-Consensus2007-SSR-4H97.67
Hordeum-LiHs-SNP-4H89.91
Hordeum-Lx41-4H118.4
Hordeum-LxHs-4H90
Hordeum-QTLConsensus-Abiotic-4H99.52
Hordeum-QTLConsensus-Agronomic-4H99.52
Hv-Consensus2006-Marcel-4H97.67
Hv-Integrated2009-4H103.6
[ Show Nearby Loci ]
Map Data
Barley, Abiotic QTL Consensus
[ Show all 10 ]
Species
Hordeum spontaneum
Hordeum vulgare
Probe
Ebmac0788
EBmac788
Reference
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceRostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527.
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark
Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb'
[ Show all 7 ]



Probe for Locus
EBmac788
Locus
Ebmac0788
EBmac788
Reference
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (TG)23
Quality = 2: Single product, relatively strong stutter bands
Diversity Index (DI) = 0.76
Type
PCR
SSR
PCR primers
5' TAACTTACTTTATATCCATGGCA 3'
5' ATGATGAGAACTCTTCACCC 3'
TAACTTACTTTATATCCATGGCA
ATGATGAGAACTCTTCACCC
SSR size
168
Amplification Conditions
1 cycle of 3 mins @ 94C, 1 min @ 66C, 1 min @ 72C, 5 cycles of 30 secs @ 94C, 30 secs @ 65C (decreasing 1C per cycle), 30 secs @ 72C, 24 cycles of 30 secs @ 94C, 30 secs @ 60C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare



Probe
Ebmac0788
Locus
Ebmac0788
Reference
ReferenceHearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391.
Type
SSR
PCR primers
TAACTTACTTTATATCCATGGCA
ATGATGAGAACTCTTCACCC

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