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GrainGenes Marker Report: Xwmc017

Locus
Xwmc017
Type
Microsatellite
Synonym
Other nameXAWM017
Chromosome
7D
Map
Wheat-Composite2004-7D124
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Probe
WMC17
Possible Orthologs
Xwmc17
Xwmc17-7A
Xwmc17-7B
wmc17
wmc17-7A



Probe for Locus
WMC17
Locus
Xwmc17-7B
[ Show all 6 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 1C6
Donor: Nickerson
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 182 bases located on 7A
AGR43. CA microsatellite. Trimed to Rsa1 fragment. (2 polymorphic loci?).
Type
PCR
SSR
PCR primers
Forward AccTgcAAgAAATTAggAAcTc at 3 length 22 bases, Tm 58
Reverse cTAgTgTTTcAAATATgTcggA at 163 length 22 bases, Tm 56
SSR size
181 bases expected, Chinese Spring
Amplification Conditions
51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence
WMC17
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06