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GrainGenes Marker Report: D1.1_ctg1058

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Locus
D1.1_ctg1058
Type
BAC_contig
Map
Wheat-Composite2004-7D22
[ Show Nearby Loci ]
Probe
CDO1400
Homology
AF192975_1e-value: 1e-04(AF192975) unknown [Oryza sativa]
BAB09169.1e-value: 4e-20(AB018113) gene_id:MFC19.8~pir||T05524~similar to unknown protein [Arabidopsis thaliana]



Probe for Locus
CDO1400
Locus
Xcdo1400-7D
[ Show all 8 ]
Synonym
SynonymHAR35_1490
HAR35_43149
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
Type
cDNA
Sequence
BE439180
BE439249
CDO1400_WHE03D10.r1
CDO1400_WHE03D10.s1
Linkage Group
2S,4,4S,5L,6
7L7S
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
1.4
PCR size
1.4
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Data Source
Sorrells, Mark E.96.07
ATMI92.09