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GrainGenes Marker Report: D1.1_ctg1075

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Locus
D1.1_ctg1075
Type
BAC_contig
Map
Wheat-Composite2004-1D87
[ Show Nearby Loci ]
Probe
CDO105



Probe for Locus
CDO105
Locus
cdo105
[ Show all 14 ]
Synonym
SynonymHAR35_10309
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
OatUni-ZAPXR
Type
cDNA
Sequence
CDO105_WHE04G03.s1
AA231700
AA231840
CDO105-3
Polymorphism
CDO105 EcoRI
CDO105 HindIII
CDO105 EcoRV
CDO105 XbaI
Linkage Group
1L [b 3L]
1L [b 3L]
Barley 5 (1H)
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
2.2
PCR size
2.2
Clone Vector
pBluSK
BS-SK-
pBS
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO105 BROWSSM autoradiogram
CDO105 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09
Kleinhofs, Andris96.05.08