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GrainGenes Marker Report: D1.1_ctg11242_2

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Locus
D1.1_ctg11242_2
Type
BAC_contig
Map
Ta-Physical-Gill-5D5.14
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Probe
CDO388
Homology
CAB72130.1e-value: 2e-04(AJ249331) heat shock protein 70 [Cucumis sativus]



Probe for Locus
CDO388
Locus
Xcdo388-1
[ Show all 53 ]
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
Type
cDNA
Sequence
BE439130
CDO388_WHE2E0091
CDO388_WHE2E0092
Polymorphism
CDO388 HindIII
Linkage Group
5L
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
1.5
PCR size
1.5
Clone Vector
pBluSK
lambda-ZapXR
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO388 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09
Gill, Bikram S.92.09