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GrainGenes Marker Report: Fr-A2

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Locus
Fr-A2
Type
Trait
Chromosome
5A
Chromosome Arm
5A
Map
Wheat-Composite2004-5A36
[ Show Nearby Loci ]
Species
Triticum aestivum
Probe
BCD508
S2269949
Associated Gene
Fr-A2 (Triticum)
Homology
T22388e-value: 4.6hypothetical protein F48F7.4 - Caenorhabditis elegans this gene~cDNA EST yk50c1.3 comes from this gene~cDNA EST yk50c1.5 comes from this gene~cDNA EST yk167b2.3 comes from this gene~cDNA EST yk167b2.5 comes from this gene~cDNA EST yk145e8.3>
Gene Class
Frost resistance
Reference
ReferenceVagujfalvi A et al. (2003) The cold-regulated transcriptional activator Cbf3 is linked to the frost-tolerance locus Fr-A2 on wheat chromosome 5A Molecular Genetics and Genomics 269:60-67.
Data Source
Wheat Gene Catalogue
Remark
Trait marker Xbcd508-5A
Nearby marker on Composite map: Xbcd508
Position on Composite map estimated by GrainGenes.



Probe for Locus
S2269949
Locus
Fr-A2
S2269949
Reference
ReferenceSieber AN et al. (2016) Copy number variation of CBF-A14 at the Fr-A2 locus determines frost tolerance in winter durum wheat Theoretical and Applied Genetics 129:1087-1097.
Type
KASP
PCR primers
5'-FAM-tail-ACTGCAGCGAG-GATTCCCA-3'
5'-AGCCACCTCTGAGATGCCGCTT-3'
5'-HEX-tail- CTACTGCAGCGAGGATTC-CCG-3'
5'-AGCCACCTCTGAGATGCCGCTT-3'
Specificity
FAM-tail primer is specific for the frost susceptible allele of Fr-A2, while the HEX-tailed allele is specific to the frost tolerant allele



Probe for Locus
BCD508
Locus
Xbcd508-1
[ Show all 21 ]
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Type
cDNA
Sequence
BE438732
BCD508_WHE1D0031
BCD508_WHE1D0032
Polymorphism
BCD508 XbaI
Linkage Group
1L, 5L
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
1.4
PCR size
1.4
Clone Vector
pBluSK
pBluSK-
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD508 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09



Gene Associated with Locus
Fr-A2 (Triticum)
Gene Class
Frost resistance
Locus
Fr-A2
S2269949
Reference
ReferenceSieber AN et al. (2016) Copy number variation of CBF-A14 at the Fr-A2 locus determines frost tolerance in winter durum wheat Theoretical and Applied Genetics 129:1087-1097.
ReferenceZhu J et al. (2014) Copy number and haplotype variation at the VRN-A1 and central FR-A2 loci are associated with frost tolerance in hexaploid wheat Theoretical and Applied Genetics 127:1183-1197.
ReferenceVagujfalvi A et al. (2003) The cold-regulated transcriptional activator Cbf3 is linked to the frost-tolerance locus Fr-A2 on wheat chromosome 5A Molecular Genetics and Genomics 269:60-67.
QTL
QFSelt.wpg-5A.1