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GrainGenes Marker Report: SukEM49j

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Locus
SukEM49j
Type
SSAP
Chromosome
7H
Map
Hordeum-SxM-SSAP-7H63
[ Show Nearby Loci ]
Map Data
Barley, Steptoe x Morex, SSAP
Probe
SukEM59
Reference
ReferenceRodriguez M et al. (2006) Integration of retrotransposons-based markers in a linkage map of barley. Molecular Breeding 17:173-184.



Probe for Locus
SukEM59
Locus
SukEM49j
[ Show all 26 ]
Reference
ReferenceLeigh F et al. (2003) Comparison of the utility of barley retrotransposon families for genetic analysis by molecular marker techniques Molecular Genetics and Genomics 269:464-474.
General Remarks
Position of retroposon-based primer in the LTR = 5' and 6 nucleotides internal to the LTR terminus
MseI primer (KeyGene code) = CTA (M59)
Type
PCR
STS
PCR primers
Sukkula-E0228 5' GGAACGTCGGCATCGGGCTG 3'
Mse1 primer M59 5' GATGAGTCCTGAGTAACTA 3'
Amplification Conditions
amplified as Vos et al Nucl Acids Res 23: 4407-4418
Source Species
Hordeum vulgare