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GrainGenes Marker Report: Yr27

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Locus
Yr27
Type
Trait
Chromosome
2B
Chromosome Arm
2B
Map
Wheat, Yr genes and QTL 2B34.4
Wheat-Composite2004-2B65
[ Show Nearby Loci ]
Map Data
Wheat, Yr genes and QTL
Species
Triticum aestivum
Probe
CDO405
Associated Gene
Yr27 (Triticum)
Homology
CAB81517.1e-value: 7e-10(AL161588) ATM-like protein [Arabidopsis thaliana]
Gene Class
Reaction to Puccinia striiformis Westend.
Data Source
Wheat Gene Catalogue
Remark
Trait marker Xcdo405-2B
Nearby marker on Composite map: Xcdo405.2
Position on Composite map estimated by GrainGenes.



Probe for Locus
CDO405
Locus
CDO405
[ Show all 18 ]
Synonym
SynonymHAR35_3670
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
OatUni-ZAPXR
Type
cDNA
Sequence
BE439061
[ Show all 6 ]
Polymorphism
CDO405 EcoRI
Durums CDO405 EcoRI
CDO405 EcoRV
Linkage Group
2S
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
1.8
PCR size
1.8
Clone Vector
pBluSK
BS-SK-
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO405 BROWS autoradiogram
CDO405 KO autoradiogram
CDO405 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09



Gene Associated with Locus
Yr27 (Triticum)
Gene Class
Reaction to Puccinia striiformis Westend.
Pathology
Stripe Rust
Locus
Yr27