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GrainGenes Probe Report: BF145935

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
BF145935
Locus
Sr25
BF145935
Reference
ReferenceLiu S et al. (2010) Diagnostic and co-dominant PCR markers for wheat stem rust resistance genes Sr25 and Sr26 Theoretical and Applied Genetics 120:691-697.
ReferenceYu LX et al. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease resistance. Stem Rust Resistance. Sr25 MAS Wheat. Marker Assisted Selection in Wheat.
Type
EST
PCR
PCR primers
BF145935-F 5'- CTT CAC CTC CAA GGA GTT CCA C -3'
BF145935-R 5'- GCG TAC CTG ATC ACC ACC TTG AAG G -3'
Amplification Conditions
Denaturing step: 94C, 4 min. Amplification step (35 cycles): 94C, 45 sec; 50C, 30 sec; 72C, 45 sec. Extension step: 72C, 7 min

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