Query (optional)   in Class  

GrainGenes Probe Report: 5S rDNA PI/PII

[ Printable Version ]  [ Submit comment/correction ]

Probe
5S rDNA PI/PII
Reference
ReferenceCox AV et al. (1992) Use of the polymerase chain reaction to detect spacer size heterogeneity in plant 5S-rRNA gene clusters and to locate such clusters in wheat (Triticum aestivum L.). Theoretical and Applied Genetics 83:684-690.
Type
PCR
STS
PCR primers
PI, 5' tgcaacac(a/g)aggacttccca
PII, 5' (t/g)t(a/c)g(t/c)gctggtatgatcgca
Amplification Conditions
Reaction mixture in 25 ul volume containing 50 mM KCl, 1.5 mM MgCl2, 10 mM Tris.HCl (pH 8.3), 0.2 mM each of dATP, dTTP, dGTP, and dCTP, 0.4 uM each primer, 2 units of Stoffel fragment, and 2.5 ul of template. PCR amplification performed at 95oC for 5 min, 30 cycles of 94oC for 55 s, 60oC for 1 s, and 72o C for 40 s, and final extension at 72oC for 5 min.
Specificity
Amplifies 5S rDNA spacer regions in monocots.
Sequence
[ Hide all but 1 of 22 ]
5SrRNA Green German
5SrRNA LA Stripe
5SrRNA HoCP 85-845
5SrRNA LCP 85-834
5SrRNA US 56-15-8
5SrRNA Djatiroto
5SrRNA IMP 9819
5SrRNA SES 84/58
5SrRNA Tainan
5SrRNA Zebra
5SrRNA IMP 3057
5SrRNA G317
5SrRNA G635
5SrRNA G931
5SrRNA NG 77-214
5SrRNA SES 288
5SrRNA Mardon
5SrRNA IMP 2886
5SrRNA Rio
5SrRNA GTX 623
5SrRNA Hulless popcorn
5SrRNA YBP76
Source Gene Class
5S ribosomal RNA
Source Species
Triticum aestivum
Data Source
Pan, Yong-Bao99.04