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GrainGenes Probe Report: AF043094

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Probe
AF043094  [ Marker Report ]
Locus
AF043094A
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (CTGT)5
Quality = 2: Single product, relatively strong stutter bands
Diversity Index (DI) = 0.38
Type
PCR
SSR
PCR primers
5' CACGGTATAAATATCCACCC 3'
5' ATGGACTCTTCTCCCTGAA 3'
SSR size
146
Amplification Conditions
1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare