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GrainGenes Probe Report: BCD1127

[Submit comment/correction] [What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
BCD1127  [ Marker Report ]
Locus
BCD1127b
[ Show all 11 ]
Synonym
Please seeBCD828
SynonymHAR35_14757
HAR35_48348
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Bar.LambZAPII
Based on surveys and mapping in Chinese Spring aneuploids and the wheat Synthetic x Opata population, this probe appears to be identical to BCD828, and should be referred to by that name. (Clare Nelson, August 1995)
Type
cDNA
Sequence
BE438744
BCD1127_WHE1H0011
BCD1127_WHE1H0012
Polymorphism
BCD1127 EcoRI
BCD1127 EcoRV
Linkage Group
3L
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
0.7
PCR size
0.7
Clone Vector
pBluSK
pBluSK-
BS-SK-
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD1127 BROWS autoradiogram
BCD1127/CSpring/EcoRV
BCD1127 KO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09

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