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GrainGenes Probe Report: BCD1130

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Probe
BCD1130  [ Marker Report ]
Locus
Xbcd1130
BCD1130
Synonym
SynonymHAR35_3715
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Bar.LambZAPII
Type
cDNA
Sequence
BE438619
BE438745
BCD1130_WHE1H0021
BCD1130_WHE1H0022
Polymorphism
BCD1130 EcoRI
BCD1130 EcoRV
Linkage Group
5S
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
1.3
PCR size
1.3
Clone Vector
pBluSK
pBluSK-
BS-SK-
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD1130 BROWS autoradiogram
BCD1130 KO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09