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GrainGenes Probe Report: BCD1235

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Probe
BCD1235  [ Marker Report ]
Locus
Xbcd1235-5A
[ Show all 18 ]
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Type
cDNA
Sequence
BE438907
BCD1235_WHE04B03.s1
Polymorphism
BCD1235 DraI
BCD1235 EcoRI
BCD1235 EcoRV
BCD1235 XbaI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
PCR size
0.7
Clone Vector
pBluSK
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD1235 KO autoradiogram
BCD1235 SO autoradiogram
Data Source
Sorrells, Mark E.96.07