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GrainGenes Probe Report: BCD127

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Probe
BCD127  [ Marker Report ]
Locus
BCD127
Xbcd127
RI052F5
HI008E8
Synonym
SynonymHAR35_16870
HAR35_44065
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Bar.LambZAPII
Type
cDNA
Sequence
BE438722
BCD127_WHE1A0031
BCD127_WHE1A0032
Polymorphism
BCD127 EcoRI
BCD127 EcoRV
BCD127 DraI
Linkage Group
3S
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
0.9
PCR size
0.9
Clone Vector
pBluSK
pBluSK-
BS-SK-
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD127 BROWS autoradiogram
BCD127 KO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09