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GrainGenes Probe Report: BCD134

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Probe
BCD134  [ Marker Report ]
Locus
Xbcd134-3
[ Show all 8 ]
Synonym
SynonymHAR35_16907
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Type
cDNA
Sequence
BE438645
BE438708
BE438799
BCD134_WHE1E0111
BCD134_WHE1E0112
Polymorphism
BCD134 XbaI
Linkage Group
3L
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
0.8
PCR size
0.8
Clone Vector
pBluSK
pBluSK-
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD134 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09