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GrainGenes Probe Report: BCD1340

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Probe
BCD1340  [ Marker Report ]
Locus
Xbcd1340
bcd1340
BCD1340
Synonym
SynonymHAR35_328
HAR35_455
HAR35_50552
HAR35_515
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Type
cDNA
Sequence
BE438656
BE438815
BCD1340_WHE1H0121
BCD1340_WHE1H0122
Polymorphism
BCD1340 EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
PCR size
1.3
Clone Vector
pBluSK
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD1340 KO autoradiogram
Data Source
Sorrells, Mark E.96.07