GrainGenes

GrainGenes Probe Report: BCD135

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	BCD135  [ Marker Report ]
Locus	[ Hide all but 1 of 14 ] BCD135a Xbcd135-2B Xbcd135 HD027F10 HI006G19 D1.1_ctg799 BCD135 Xbcd135A Xbcd135B BCD135B Xbcd135-2D bcd135 BCD135A bcd135-2B
Synonym	Synonym HAR35_18261 HAR35_18262 HAR35_47679
Reference	Heun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks	Lambda ZAP II library Bar.LambZAPII [c] Bar.LambZAPII
Type	cDNA
Sequence	BE438835 [ Show all 9 ]
Polymorphism	BCD135 EcoRI
Linkage Group	2L
Insert Enzyme	EcoRI
Source Species	Hordeum vulgare
Source Germplasm	Willis
Source Tissue	etiolated leaf
Insert Size	0.7
PCR size	0.7
Clone Vector	pBluSK pBluSK- BS-SK-
Excision Enzyme	EcoRI
Vector PCR primers	M13forward M13reverse
Vector Amplification	94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic	Ampicillin
Clone Location	Sorrells, Mark E. GrainGenes Probe Repository
Clone Authority	Sorrells, Mark E.
Image	BCD135 BROWS autoradiogram BCD135 KO autoradiogram
Data Source	Sorrells, Mark E. 96.07 ATMI 92.09

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