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GrainGenes Probe Report: BCD141

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
BCD141  [ Marker Report ]
Locus
Xbcd141-3A
Xbcd141-3B
RI038D14
Xbcd141
bcd141
Synonym
SynonymHAR35_17539
HAR35_17540
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Type
cDNA
Sequence
BE438875
BE438897
BCD141_WHE03G11.r1
BCD141_WHE03G11.s1
Polymorphism
BCD141 EcoRV
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
PCR size
1
Clone Vector
pBluSK
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD141 SO autoradiogram
Data Source
Sorrells, Mark E.96.07

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