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GrainGenes Probe Report: BCD1434

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Probe
BCD1434  [ Marker Report ]
Locus
BCD1434
[ Show all 19 ]
Synonym
SynonymHAR35_16734
HAR35_16735
HAR35_42959
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Bar.LambZAPII
Type
cDNA
Sequence
BE438866
[ Show all 6 ]
Polymorphism
BCD1434 EcoRI
BCD1434 XbaI
BCD1434 EcoRV
BCD1434 HindIII
Linkage Group
1S
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
1.2
PCR size
1.2
Clone Vector
pBluSK
pBluSK-
BS-SK-
lambda_Zap
pBS
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
m13reverse 5'AACAGCTATGACCATG
m13forward 5'GTAAAACGACGGCCACT
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD1434 BROWS autoradiogram
BCD1434 SO autoradiogram
pBCD1434
Data Source
Sorrells, Mark E.96.07
ATMI92.09
Gill, Bikram S.92.09