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GrainGenes Probe Report: BCD15

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Probe
BCD15  [ Marker Report ]
Locus
BCD15
[ Show all 21 ]
Synonym
SynonymHAR35_1280
HAR35_1282
HAR35_15304
HAR35_32773
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Type
cDNA
Sequence
BE438607
BE438662
BE438747
BCD15_WHE1A0011
BCD15_WHE1A0012
Polymorphism
BCD15 EcoRI
Linkage Group
4L
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
1.6
PCR size
1.6
Clone Vector
pBluSK
pBluSK-
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD15 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09