Query (optional)   in Class  

GrainGenes Probe Report: BCD1510

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
BCD1510  [ Marker Report ]
Locus
Xbcd1510-6
[ Show all 9 ]
Synonym
Other nameBCD880
Hybridization_homologue: The two clones were isolated independently but exhibit the same RFLP patterns when hybridized to DNA from test genotypes, and thus are closely related in sequence.
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Type
cDNA
Polymorphism
BCD1510 EcoRI
BCD1510 EcoRV
BCD1510 HindIII
BCD1510 XbaI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
PCR size
0.9
Clone Vector
pBluSK
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
Clone Authority
Sorrells, Mark E.
Image
BCD1510 SO autoradiogram
Data Source
Sorrells, Mark E.96.07

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.