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GrainGenes Probe Report: BCD1555

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Probe
BCD1555  [ Marker Report ]
Locus
bcd1555
[ Show all 16 ]
Synonym
SynonymHAR35_17539
HAR35_17540
HAR35_2907
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Type
cDNA
Sequence
BE438991
BE439020
BCD1555_WHE05C08.s1
Polymorphism
BCD1555 DraI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
PCR size
1.2
Clone Vector
pBluSK
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD1555 KO autoradiogram
BCD1555 SO autoradiogram
Data Source
Sorrells, Mark E.96.07