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GrainGenes Probe Report: BCD1643

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Probe
BCD1643  [ Marker Report ]
Locus
bcd1643a
[ Show all 14 ]
Synonym
Other nameBC1643
SynonymHAR35_695
HAR35_696
HAR35_699
Reference
ReferenceWight CP et al. (2004) Discovery, localization, and sequence characterization of molecular markers for the crown rust resistance genes Pc38, Pc39, and Pc48 in cultivated oat (Avena sativa L.) Molecular Breeding 14:349-361.
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Type
cDNA
Sequence
BE438817
BE438838
BCD1643_WHE2A0042
BCD1643_WHE2A0041
CN180783
Polymorphism
BCD1643 EcoRV
BCD1643 EcoRI
BCD1643 DraI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
PCR size
1.3
Clone Vector
pBluSK
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD1643 KO autoradiogram
Data Source
Sorrells, Mark E.96.07
Sorrells, Mark E.94.10.31