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GrainGenes Probe Report: BCD1734

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Probe
BCD1734  [ Marker Report ]
Locus
Xbcd1734
bcd1734
BCD1734
Synonym
SynonymHAR35_4567
HAR35_4568
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Type
cDNA
Sequence
BE438993
BE439022
BCD1734_WHE05D06.s1
Polymorphism
BCD1734 EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
PCR size
0.8
Clone Vector
pBluSK
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD1734 KO autoradiogram
Data Source
Sorrells, Mark E.96.07